By using Reactive oxygen species assay and mitochondrial membrane potential assaywhich help to study the effect of nanoparticles on cells( confirm the toxicity of this Nanoparticles) So I need to write to me literature review to explain that there are no studies explain the oxidative stress of L- or D- tyrosine and tryptophan with AgNps and with AuNPs to explain which type of amino acid is more toxic or less toxic (L- amino acid or D- amino acid coated with metal nanoparticles ) on macrophages (J774)and cause that I did this experiment to determine that Preferred language style Australian English Revision instructions SUPPORT : Me Feb 19 2018 06:06 PM The order 268501642 needs to be revised according to the following comments: This is my initial massage :No need to add revision point cause he did not do the basic thing. It is not literature review even the topic is wrong which mean he did not give 5 second to read what I want . By using Reactive oxygen species assay and mitochondrial membrane potential assay which help to study the effect of nanoparticles on cells( confirm the toxicity of this Nanoparticles) So I need to write to me literature review to explain that there are no studies explain the oxidative stress of L- or D- tyrosine and tryptophan with AgNPs (silver nanoparticles) and with AuNPs ( gold nanoparticles)to explain which type of amino acid is best to maintain the oxidative stress in the cells (L- amino acid or D- amino acid coated with metal nanoparticles ) on macrophages (J774)and cause that I did this experiment to determine that

Effect of silver nanoparticles on L- and D- tyrosine/tryptophan (HSA)

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Effect of silver nanoparticles on L- and D- tyrosine/tryptophan (HSA)
In a broad spectrum, the use of Silver nanoparticles as antibacterial agents has become more common in the consumer products. Therefore, Silver nanoparticles are used to control the quantity of human serum albumin binding. According to Basiuk, Vladimir A, and Elena V. Basiuk (2015), the review shows that the interaction between of L- or D- tyrosine and tryptophan (HSA) and NPs was a lower procedure driven by hydrophobic power and suppressed by NP surface charge incorporation with shell-center composition
The research depicts different methods applied to demonstrate almost the same thing in the hydrophilic components and cholesterol. Undeniably, a constant nanoparticle is in the form of aqueous solution because it is a non-covalent bond from intermolecular combination with the hydrophobic components (RAHMAN 2016, p.32). Consequently, the morphologic compositions are structured by the chemical conformation of CHP. As a result, an increasing of DS cholesterol stimulated the lowering of nanoparticles dimension due to the provoked chances of interaction with L- or D- tyrosine and tryptophan pendant groups. Therefore, L- or D- tyrosine and tryptophan predominated on the structure surface for a while simultaneously displaced by lowering its affinity level (RAHMAN 2016, p.32). Consequently, the L-tyrosine acquire lower affinity.
Furthermore, in the research, it is noted that oxidative stress is linked to the aging process. Free radicals can have a toxic effect on the cells, as the cells are only able to overcome small amounts of free radical to retain their state (Davies et al. 2015). When there is moderate oxidative stress, then it can trigger apoptosis, and increased reactive oxygen species (ROS) which lead to ROS are associated with the loss of bone mass in the aging process. Oxidized amino acids may act as pathogens in the age-related bone loss.

In conclusion, it is revealed to the researchers that there are no proven studies on the L-and D- tyrosine and tryptophan. On the other side, the mixture of nanoparticles and L- or D- tyrosine and tryptophan interface was driven by a hydrophobic structure. After combining, HSA was rapidly adsorbed to the silver nanoparticle surface by hydrophobic forces from cholesterol fragments. The nanoparticles complexation and release of drug affects particle surface charge. Notably, adsorbed HSA proceeded to be withdrawn from the cholesterol due to the hydrophobic reaction. In the process, the adsorbed HSA slowly moved into the centre, however, affecting with polysaccharide bond.


References

BASIUK, V. A., & BASIUK, E. V. (2015). Green processes for nanotechnology: from inorganic to bioinspired nanomaterials. http://public.eblib.com/choice/publicfullrecord.aspx?p=3108766.
RAHMAN, M., LAURENT, S., TAWIL, N., YAHIA, L., & MAHMOUDI, M. (n.d.) (2016). Protein-Nanoparticle Interactions [recurso electrónico] The Bio-Nano Interface.